Creative Proteomics announced the launch of methylation analysis, a useful alternative to explore life activities and combat diseases.
Methylation modifications can occur at different amino acid positions, or more than one methylation modification can occur at the same amino acid position. Therefore, for the study of protein function, determining the methylation site is the primary task of studying its function. Through a variety of molecular biology experimental techniques, combined with proteomics and other methods, understand the function of protein methylation, and further explore its interaction with different PTMs, and then understand its role in cell signaling pathways, animals development and disease treatment, which will help us to better understand the laws of prokaryotic life activities, and blocking and interfering with key links will also provide better scientific basis and measures for combating related diseases.
Methylation refers to the process of catalytically transferring methyl groups from active methyl compounds (such as S-adenosylmethionine) to other compounds. Various methyl compounds can be formed, or certain proteins or nucleic acids can be chemically modified to form methylated products. In biological systems, methylation is catalyzed by enzymes. This methylation involves heavy metal modification, gene expression regulation, protein function regulation, and ribonucleic acid (RNA) processing.
Methylation is an important modification of proteins and nucleic acids, regulates the expression and shutdown of genes, and is closely related to many diseases such as cancer, aging, and dementia. It is one of the important research contents of epigenetics. The most common methylation modifications are DNA methylation and histone methylation.
Methylation includes DNA methylation or protein methylation
a. DNA methylation. Vertebrate DNA methylation generally occurs at the CpG site (cytosine-phosphate-guanine site, that is, the site of cytosine immediately after guanine in the DNA sequence). The conversion of cytosine to 5-methylcytosine is catalyzed by DNA methyltransferase. About 80% -90% of CpG sites in human genes have been methylated, but in certain regions, such as CpG islands rich in cytosine and guanine, are not methylated. This is related to the promoter in 56% of mammalian genes, including all widely expressed genes. 1% -2% of the human genome is the CpG group, and CpG methylation is inversely proportional to transcriptional activity.
b. Protein methylation. Protein methylation generally refers to the methylation of arginine or lysine in protein sequences. Arginine can be methylated once (called monomethylarginine) or twice (arginine methyltransferases (PRMTs) to transfer two methyl groups to the same nitrogen at the end of the arginine polypeptide. Atomically, it becomes asymmetric methylarginine, or a methyl group is added to each nitrogen end to become symmetrical dimethylarginine.) Lysine can be methylated once, twice or three times by lysine transferase. Among histones, protein methylation is the most studied. Under the catalysis of histone transferase, the methyl group of S-adenosylmethionine is transferred to histone. Some histone residues can inhibit or activate gene expression through methylation, thereby forming epigenetic. Protein methylation is a form of post-translational modification.
(1) Methylation-specific PCR (MSP)
(2) Bisulfite sequencing PCR (BSP)
(3) High Resolution Melting (HRM)
(4) Direct genome sequencing
About Creative Proteomics
Methylation analysis methods include HPLC-UV, LC-MS, ELISA-Based Methods, LINE-1+ Pyrosequencing, Levels of LINE-1 methylation and so on. Other methods include AFLP and RFLP, LUMA, methylation sequencing with NGS, NGS causes overall profiling of methylation modes at single-base resolution across the whole genome, or in targeted epigenetic districts of interest. Creative Proteomics have a strict workflow to analysis methylation to meet research requirements.
Pronalyse is a newly established division of Creative Proteomics, which is an integrated CRO company with rich experience in providing drug development service for over 10 years. The name Pronalyse is derived from ‘professional’ and ‘analyze’. It is dedicated in providing professional analysis service in biopharmaceutical development, with the most advanced technology, within the shortest turnaround time, and at the most competitive price.