New York, USA – July 30, 2020 – Lifeasible, a global company specialized in plant biotechnology and plant molecular biology provides high-quality analytical services for functional characterization of plant genes, as well as manufacturing of plant products. With solid biochemical and cytological backgrounds, and years of experience in plant cell line/tissue sample generation, Lifeasible now provides high quality service that covers every step of the FISH assay in plant system.
Fluorescence in situ hybridization (FISH) is a valuable cytogenetic technique for the detection and localization of specific DNA or RNA sequences on chromosomes. Typically, this technique can be used to define spatiotemporal expression patterns of target genes. Using fluorescent DNA probes with sequences complementary to the location of the chromosome of interest, gene expression patterns can be easily detected by fluorescence microscopy and further quantitatively analyzed. In addition, improved FISH analysis also allows simultaneous observation of multiple genes by labeling different sequence fragments with different fluorophores.
In plant systems, FISH analysis has been used in a variety of crops, including wheat, rye, cucumber and melon. Based on the information on Lifeasible’s official website, Lifeasible’s services cover every step of the FISH assay in the plant system:
• Probe designing and construction: aside from commercially available probes, Lifeasible also provides customized probes to meet special needs
• Sample preparation: chromosome, cell line and tissue samples
• Probe/target DNA denaturation and hybridization: optimized protocol ensures better chromosome spreading, and more efficient hybridization
• Result acquisition, interpretation and reporting: superior microscope system that can obtain high quality images, which allows for detection of genes even with low copy numbers
“As a prominent plant biotechnology company, we provide high quality services for gene copy number analysis in the plant system. Aside from a full array of experimental reagents, kits and instruments, we also offer customized protocols to meet the specialized requirements from each project,” said the representative speaker from Lifeasible.
Visit https://www.lifeasible.com/custom-solutions/plant/analytical-services/gene-copy-number-analysis/the-fish-assay-in-plants to know more detailed information about FISH Assay in Plants offered by Lifeasible.