Because of the high specific catalytic activity of the enzyme, the enzyme activity can be determined by determining the concentration of the corresponding substrate or product, or by changing the concentration of a reaction product or reactant. Electrochemical and photophysical methods are commonly used to determine with the absorbance of reactants or products by UV or fluorescence spectroscopy. If the product of enzyme reaction produces gas, it is usable to use manometry for determination. If the acid is produced during the reaction, the electrochemical method can used. Isotope labeled substrates can be used to determine the concentration of substrate and the activity of the enzyme is calculated by the radiochemical method. Some of the stable enzymes can also be detected by high performance liquid chromatography.
Here are a few details of enzyme activity by Creative Enzymes for determination on Phosphorus Transferases, Pyruvate Kinase and Glucokinase.
This is an enzyme that catalyzes phosphate group from a donor to an acceptor. According to the different acceptor groups, it is divided into the following categories: alcohol group; carboxyl group; nitrogen group; phosphoryl group; etc.
The enzyme is adapted to a wide variety of substrates, ranging from small organic molecules to large proteins, which lead to complex determination of enzyme activity. In addition, many kinases have high homology in the amino acid sequence, which makes the activity assay interfered with the different types of kinases in the mixed enzyme systems. At the same time, interest in research has increased significantly with the discovery of the key role of phosphotransferase in cell proliferation and signal transduction.
Pyruvate kinase is a class of enzymes that catalyze phosphate groups from phosphoenolpyruvate (PEP) to ADP to produce a molecule of pyruvate and a molecule of ATP.
Pyruvate kinase is the last step in the process of glycolysis. The enzyme catalyzes the irreversible phosphorylation of ADP at the expense of phosphoenolpyruvate (PEP), resulting in pyruvate and ATP.
Glucokinase is an inducible enzyme. Insulin can induce the synthesis, the increased glucose concentration in plasma can also induce its synthesis. There is no allosteric site of 6- phosphate glucose molecule, and it is not influenced by 6- phosphate glucose. 6- phosphate glucose has low affinity to glucose. The Km value is about 10mmol/L. It is highly specific for glucose.
Company Name: Creative Enzymes
Contact Person: Jack Forst
Address:45-1 Ramsey Road
State: New York
Country: United States