Apoptosis is a form of programmed cell death to remove unwanted, damaged, or senescent cells from tissues. In normal cells, the negative phospholipids reside on the inner side of the cellular membrane while the outer surface of the membrane is occupied by uncharged phospholipids (PS). After a cell has entered apoptosis, the negatively charged PS are transported from the inner to the outer leaflet of the plasma membrane, thus exposing PS to the external cellular environment. The human anticoagulant, Annexin V, is a 35–36 kDa Ca2+-dependent phospholipid-binding protein that has a high affinity for PS. Annexin V labeled with a fluorophore or biotin can identify apoptotic cells by binding to PS exposed on the outer leaflet.
Propidium iodide (PI) is a membrane-impermeant DNA-binding dye that is commonly used to selectively stain dead cells in a cell population. PI is excluded by live cells and early apoptotic cells, but stains necrotic and late apoptotic cells with compromised membrane integrity. PI can be excited by the 488, 532, or 546 nm laser lines, and emits red ﬂuorescence.
Based on Abbkine strong cell platform and advanced technology, Abbkine Annexin V Apoptosis Detection kits are generated at the right moment. Abbkine Annexin V Apoptosis Detection kit contains Annexin V labeled with Abbkine proprietary AbFluor™ dyes, which allows the identification and quantitation of apoptotic cells by flow cytometry or fluorescence microscopy. Simultaneous staining of cells with AbFluor™ dyes and propidium iodide (PI) allows the discrimination of intact cells, early apoptotic and late apoptotic or necrotic cells.
Please see the below list for details
|Annexin V-AbFluor™ 405 Apoptosis Detection kit||KTA0001||Annexin V- AbFluor™ 405: Abs/Em = 404/431 nm; PI: Abs/Em = 535/617 nm (with DNA)|
|Annexin V-AbFluor™ 488 Apoptosis Detection kit||KTA0002||Annexin V- AbFluor™ 488: Abs/Em = 491/517 nm; PI: Abs/Em = 535/617 nm (with DNA)|
|Annexin V-AbFluor™ 555 Apoptosis Detection kit||KTA0003||Annexin V- AbFluor™ 555: Abs/Em = 555/565 nm|
|Annexin V-AbFluor™ 647 Apoptosis Detection kit||KTA0004||Annexin V- AbFluor™ 647: Abs/Em = 650/665 nm; PI: Abs/Em = 535/617 nm (with DNA)|
Abbkine offers a variety of Annexin V conjugates labeled with our outstanding series of AbFluor™ dyes. AbFluor™ dyes have equal or much better labeling performance than other fluorescent labeling dyes such as FITC, TRITC, Cy® dyes, Alexa Fluor® and Dylight® labeling dyes by having combined advantages in brightness, photostability, specificity and novel features ideal for in vivo imaging and flow cytometry.
Take Annexin V-AbFluor™ 488 Apoptosis Detection kit for example. The typical data is as below.
|Hela cells were induced with camptothecin for 24 hours and stained with Annexin V- AbFluor™ 488 Apoptosis Detection Kit. The combination of AbFluor™ 488 and propidium iodide allows for the distinction between early apoptotic cells (Annexin V- AbFluor™ 488 positive), late apoptotic and/or necrotic cells (Annexin V- AbFluor™ 488 and propidium iodide positive), and viable cells (unstained).|
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