New York, Jul 30th, 2018 – Creative Bioarray, a reliable cutting-edge biotechnology supplier, offering a full range of chromosome probes. The CABR chromosome probes we provided include a portfolio of products, such as whole chromosome painting probes, centromere probes, subtelomere specific probes, satellite enumeration probes etc. With all of these offering, Creative Bioarray is dedicated to detecting the gene amplification, deletion, and translocation, as well as covering each human chromosome to satisfy related study needs.
Fluorescence in situ hybridization is an efficient method for identifying specific nucleic acid sequences in metaphase or interphase cells visually. The accuracy, repeatability and reliability of FISH analysis depend primarily on the specificity and sensitivity of the probe used and the efficiency of hybridization detection. The probe can be labeled with a fluorescent label (direct method) or with a non-fluorescent molecule. There are various types of DNA probes available for FISH, including CEP (Chromosome Counting Probe), LSI (Gene-Specific Labeling Probe), Telomere-Specific DNA Probe, WCP (Whole Chromosome Mapping Probe) etc. A particular application of FISH is the use of whole-chromosome probes, called chromosome painting. WCP is a collection of DNA fragments that hybridize to different sequences along the entire length of the chromosome. Chromosomal coatings have been getting more application in the identification of complex chromosomal rearrangements (including de novo rearrangements) and small marker chromosomes, particularly in clinical and cancer cytogenetics.
The principle of fluorescence in situ hybridization：
. The basic elements include DNA probes and target sequences.
. The DNA probe is indirectly labeled with a hapten or directly labeled by incorporation of a fluorophore before hybridization.
. Obtain single-stranded DNA via denaturation of the labeled probe and the target DNA.
. Combining labeled probe and target DNA, which allows annealing of the complementary DNA sequence.
. If the probe is indirectly labeled, an additional step is required to observe the non-fluorescent hapten with the enzyme or immunodetection system. Finally, the signal will be evaluated by fluorescence microscopy.
Application of FISH Chromosome Probes
Different types of FISH probes can detect different chromosomal aberrations. Probes specific to a particular locus can determine gene fusion in adults, aneuploidy or abnormal chromosome numbers, and loss of chromosomal regions or entire chromosomes. The resolution of FISH is 50 times higher than that of the conventional Giemsa staining method for detecting genetic diseases.
FISH is widely used to detect chromosomal abnormalities associated with cancer. It also has a higher sensitivity in detecting cancer. Meanwhile, FISH is also used to detect pathogens within tissues. Genus and species-specific oligonucleotide probes have been used to identify pathogenic microorganisms in blood culture.
‘Chromosome probe is essential for improving chromosome related studies,’ said Donghai, Chen, business director at Creative Bioarray, ‘We have collected a full range of FISH probes knowledge and enhanced high-quality probe products to make our customers get more professional human chromosome to match study needs best and achieve powerful research performance.’
About Creative Bioarray
Founded in 2005, Creative Bioarray provides customers with high quality products including FISH probes (such as Whole Chromosome Painting Probes), tissue array and microorganisms to greatly enhance experiments performance and drive innovation and standards in science. Creative Bioarray is a well-recognized biology industry leader with more than 10 years experience, millions of active users worldwide.
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