Abbkine Releases Protein Quantification Kit (BCA Assay)

“Abbkine Protein Quantification Kit (BCA Assay) standard curve”
Abbkine BCA Protein Assay Kit provides a simple, rapid, detergent-compatible procedure for the detection and quantitation of total protein.

Product name: Protein Quantification Kit (BCA Assay)

Application notes: Abbkine BCA Protein Assay Kit provides a simple, rapid, detergent-compatible procedure for the colorimetric detection and quantitation of total protein. The method utilizes the reduction of the copper cation(Cu2+) to the cuprous cation(Cu+) by protein in an alkaline medium. The generated Cu+ forms an intensely purple-colored complex with the bicinchoninic acid(BSA) with a very strong absorbance at 562nm. The intensity of the water-soluble complex is proportional to the amount of protein in the sample. The BCA Protein Assay is suitable for measuring protein concentration in the range of 50-1000µg/ml.

Detection method: Colorimetric

Kit components:

• BCA Reagent A (2x)

• BCA Reagent B

• BSA Standard

Features & Benefits:

• Time saving — much easier and four times faster than the classical Lowry method.

• Sensitive — detect down to 25 µg/ml and the minimum amount of detection protein reaches 0.5μg.

• Compatible — unaffected by most ionic and nonionic detergents. Can be compatible with up to 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80 in the sample.

• Good linearity — linear working range from 50 to 1000 µg/ml.

• Excellent uniformity — exhibits less protein-to-protein variation than dye-binding methods.

Calibration range: 50-1000 µg/ml

Limit of detection: 25 µg/ml

Size and Price:

Size Price
500T $45
2000T $100
5000T $180

More information, please click KTD3001.

Media Contact
Company Name: Abbkine Scientific
Contact Person: Media Relations
Email: Send Email
Phone: +86-27-59716789
Address:Bldg 1, Harbour of Technology Times, No.35, Optical Valley Ave
City: Wuhan
State: Hubei
Country: China
Website: https://www.abbkine.com/