How to choose an Epitope Tag antibody?

Abbkine epitope tag antibodies not only include highly specific monoclonal and polyclonal antibodies of His tag, GST tag, S tag, HA tag, DDDDK tag, Myc tag, MBP Tag and mCherry tag conjugated, but also contain Biotin, HRP, AbFluor™ dyes, agarose and magnetic beads conjugated.

Epitope tags are frequently used with recombinant protein from E. coli, yeast or mammalian cell cultures to allow researchers to selectively extract a target protein from the endogenous samples. Abbkine not only provides such as His tag, GST tag, S tag, HA tag, DDDDK tag, Myc tag, MBP Tag and mCherry tag monoclonal and polyclonal antibodies, but also offers Biotin, HRP, AbFluor™ dyes, agarose and magnetic beads conjugated antibodies.

Below I will overview in different tags for you.

1. Anti-DDDDK Tag antibodies

Product Name  Cat. No Applications
Anti-DDDDK Tag Mouse Mab (1B10) A02010 WB, IF, IP
Anti-DDDDK Tag Mouse Mab (1B10), AbFluor™ 350 Conjugated A02010A350 IF
Anti-DDDDK Tag Mouse Mab (1B10), AbFluor™ 405 Conjugated A02010A405 IF
Anti-DDDDK Tag Mouse Mab (1B10), AbFluor™ 488 Conjugated A02010A488 IF
Anti-DDDDK Tag Mouse Mab (1B10), AbFluor™ 555 Conjugated A02010A555 IF
Anti-DDDDK Tag Mouse Mab (1B10), AbFluor™ 594 Conjugated A02010A594 IF
Anti-DDDDK Tag Mouse Mab (1B10), AbFluor™ 647 Conjugated A02010A647 IF
Anti-DDDDK Tag Mouse Mab (1B10), AbFluor™ 680 Conjugated A02010A680 IF
Anti-DDDDK Tag Mouse Mab (1B10), Cy3 Conjugated A02010CY3 IF
Anti-DDDDK Tag Mouse Mab (1B10), Cy5 Conjugated A02010CY5 IF
Anti-DDDDK Tag Mouse Mab (1B10), FITC Conjugated A02010FIT IF
Anti-DDDDK Tag Mouse Mab (1B10), Agarose A02010AGB IP
Anti-DDDDK Tag Mouse Mab (1B10), Magnetic Beads A02010MGB IP
Anti-DDDDK Tag Rabbit Pab A02011 WB
Anti-DDDDK Tag Rabbit Pab, HRP Conjugated A02011HRP WB
HRP Conjugated Anti-DDDDK Tag Mouse Mab (1B10) A02015 WB

2. Anti-HA Tag antibodies

Product Name  Cat. No Applications
Anti-HA Tag Mouse Mab (4F6) A02040 WB, IF, IP
Anti-HA Tag Mouse Mab (4F6), AbFluor™ 350 Conjugated A02040A350 IF
Anti-HA Tag Mouse Mab (4F6), AbFluor™ 405 Conjugated A02040A405 IF
Anti-HA Tag Mouse Mab (4F6), AbFluor™ 488 Conjugated A02040A488 IF
Anti-HA Tag Mouse Mab (4F6), AbFluor™ 555 Conjugated A02040A555 IF
Anti-HA Tag Mouse Mab (4F6), AbFluor™ 594 Conjugated A02040A594 IF
Anti-HA Tag Mouse Mab (4F6), AbFluor™ 647 Conjugated A02040A647 IF
Anti-HA Tag Mouse Mab (4F6), AbFluor™ 680 Conjugated A02040A680 IF
Anti-HA Tag Mouse Mab (4F6), Cy3 Conjugated A02040CY3 IF
Anti-HA Tag Mouse Mab (4F6), Cy5 Conjugated A02040CY5 IF
Anti-HA Tag Mouse Mab (4F6), FITC Conjugated A02040FIT IF
Anti-HA Tag Mouse Mab (4F6), Agarose A02040AGB IP
Anti-HA Tag Mouse Mab (4F6), Magnetic Beads A02040MGB IP
Anti-HA Tag Rabbit Pab A02041 WB
Anti-HA Tag Rabbit Pab, HRP Conjugated A02041HRP WB
HRP Conjugated Anti-HA Tag Mouse Mab (4F6) A02045 WB

3. Anti-His Tag antibodies

Product Name  Cat. No Applications
Anti-His Tag Mouse Mab (5C3) A02050 WB, IF, IP
Anti-His Tag Mouse Mab (5C3), AbFluor™ 350 Conjugated A02050A350 IF
Anti-His Tag Mouse Mab (5C3), AbFluor™ 405 Conjugated A02050A405 IF
Anti-His Tag Mouse Mab (5C3), AbFluor™ 488 Conjugated A02050A488 IF
Anti-His Tag Mouse Mab (5C3), AbFluor™ 555 Conjugated A02050A555 IF
Anti-His Tag Mouse Mab (5C3), AbFluor™ 594 Conjugated A02050A594 IF
Anti-His Tag Mouse Mab (5C3), AbFluor™ 647 Conjugated A02050A647 IF
Anti-His Tag Mouse Mab (5C3), AbFluor™ 680 Conjugated A02050A680 IF
Anti-His Tag Mouse Mab (5C3), Cy3 Conjugated A02050CY3 IF
Anti-His Tag Mouse Mab (5C3), Cy5 Conjugated A02050CY5 IF
Anti-His Tag Mouse Mab (5C3), FITC Conjugated A02050FIT IF
Anti-His Tag Mouse Mab (5C3), Agarose A02050AGB IP
Anti-His Tag Mouse Mab (5C3), Magnetic Beads A02050MGB IP
Anti-His Tag Rabbit Pab A02051 WB
Anti-His Tag Rabbit Pab, HRP Conjugated A02051HRP WB
HRP Conjugated Anti-His Tag Mouse Mab (5C3) A02055 WB

4. Anti-V5 Tag antibodies

Product Name  Cat. No Applications
Anti-V5 Tag Mouse Mab (11D5) A02170 WB, IF, IP
Anti-V5 Tag Mouse Mab (11D5), AbFluor™ 350 Conjugated A02170A350 IF
Anti-V5 Tag Mouse Mab (11D5), AbFluor™ 405 Conjugated A02170A405 IF
Anti-V5 Tag Mouse Mab (11D5), AbFluor™ 488 Conjugated A02170A488 IF
Anti-V5 Tag Mouse Mab (11D5), AbFluor™ 555 Conjugated A02170A555 IF
Anti-V5 Tag Mouse Mab (11D5), AbFluor™ 594 Conjugated A02170A594 IF
Anti-V5 Tag Mouse Mab (11D5), AbFluor™ 647 Conjugated A02170A647 IF
Anti-V5 Tag Mouse Mab (11D5), AbFluor™ 680 Conjugated A02170A680 IF
Anti-V5 Tag Mouse Mab (11D5), Cy3 Conjugated A02170CY3 IF
Anti-V5 Tag Mouse Mab (11D5), Cy5 Conjugated A02170CY5 IF
Anti-V5 Tag Mouse Mab (11D5), FITC Conjugated A02170FIT IF
Anti-V5 Tag Mouse Mab (11D5), HRP Conjugated A02170HRP WB
Anti-V5 Tag Mouse Mab (11D5), Agarose A02170AGB IP
Anti-V5 Tag Mouse Mab (11D5), Magnetic Beads A02170MGB IP

5. Anti-Myc Tag antibodies

Product Name  Cat. No Applications
Anti-Myc Tag Mouse Mab (2D5) A02060 WB, IF, IP
Anti-Myc Tag Mouse Mab (2D5), AbFluor™ 350 Conjugated A02060A350 IF
Anti-Myc Tag Mouse Mab (2D5), AbFluor™ 405 Conjugated A02060A405 IF
Anti-Myc Tag Mouse Mab (2D5), AbFluor™ 488 Conjugated A02060A488 IF
Anti-Myc Tag Mouse Mab (2D5), AbFluor™ 555 Conjugated A02060A555 IF
Anti-Myc Tag Mouse Mab (2D5), AbFluor™ 594 Conjugated A02060A594 IF
Anti-Myc Tag Mouse Mab (2D5), AbFluor™ 647 Conjugated A02060A647 IF
Anti-Myc Tag Mouse Mab (2D5), AbFluor™ 680 Conjugated A02060A680 IF
Anti-Myc Tag Mouse Mab (2D5), Cy3 Conjugated A02060CY3 IF
Anti-Myc Tag Mouse Mab (2D5), Cy5 Conjugated A02060CY5 IF
Anti-Myc Tag Mouse Mab (2D5), FITC Conjugated A02060FIT IF
Anti-Myc Tag Mouse Mab (2D5), Agarose A02060AGB IP
Anti-Myc Tag Mouse Mab (2D5), Magnetic Beads A02060MGB IP
Anti-Myc Tag Rabbit Pab A02061 WB
Anti-Myc Tag Rabbit Pab, HRP Conjugated A02061HRP WB
HRP Conjugated Anti-Myc Tag Mouse Mab (2D5) A02065 WB

6. Anti-VSV-G Tag antibodies

Product Name  Cat. No Applications
Anti-VSV-G-Tag Mouse Mab (14D2) A02180 WB, IF, IP
Anti-VSV-G-Tag Mouse Mab (14D2), AbFluor™ 350 Conjugated A02180A350 IF
Anti-VSV-G-Tag Mouse Mab (14D2), AbFluor™ 405 Conjugated A02180A405 IF
Anti-VSV-G-Tag Mouse Mab (14D2), AbFluor™ 488 Conjugated A02180A488 IF
Anti-VSV-G-Tag Mouse Mab (14D2), AbFluor™ 555 Conjugated A02180A555 IF
Anti-VSV-G-Tag Mouse Mab (14D2), AbFluor™ 594 Conjugated A02180A594 IF
Anti-VSV-G-Tag Mouse Mab (14D2), AbFluor™ 647 Conjugated A02180A647 IF
Anti-VSV-G-Tag Mouse Mab (14D2), AbFluor™ 680 Conjugated A02180A680 IF
Anti-VSV-G-Tag Mouse Mab (14D2), Cy3 Conjugated A02180CY3 IF
Anti-VSV-G-Tag Mouse Mab (14D2), Cy5 Conjugated A02180CY5 IF
Anti-VSV-G-Tag Mouse Mab (14D2), FITC Conjugated A02180FIT IF
Anti-VSV-G-Tag Mouse Mab (14D2), HRP Conjugated A02180HRP WB
Anti-VSV-G-Tag Mouse Mab (14D2), Agarose A02180AGB IP
Anti-VSV-G-Tag Mouse Mab (14D2), Magnetic Beads A02180MGB IP

7. Other Tag antibodies

Product Name  Cat. No Applications
Anti-GFP Tag Mouse Mab (3D3) A02020 WB, IP
Anti-GFP Tag Mouse Mab (3D3), HRP Conjugated A02020HRP WB
Anti-GFP Tag Rabbit Pab A02021 WB
Anti-GFP Tag Rabbit Pab, HRP Conjugated A02021HRP WB
Anti-GST Tag Mouse Mab (2A8) A02030 WB
Anti-GST Tag Mouse Mab (2A8), HRP Conjugated A02030HRP WB
Anti-MBP Tag Mouse Mab (9Y5) A02070 WB
Anti-MBP Tag Mouse Mab (9Y5), HRP Conjugated A02070HRP WB
Anti-mCherry Tag Mouse Mab (9D3) A02080 WB, IF
Anti-mCherry Tag Mouse Mab (9D3), HRP Conjugated A02080HRP WB
Anti-E2 Tag Mouse Mab (12T4) A02090 WB
Anti-E2 Tag Mouse Mab (12T4), HRP Conjugated A02090HRP WB
Anti-HSV Tag Mouse Mab (16T2) A02100 WB
Anti-HSV Tag Mouse Mab (16T2), HRP Conjugated A02100HRP WB
Anti-KT3 Tag Mouse Mab (14D8) A02110 WB
Anti-KT3 Tag Mouse Mab (14D8), HRP Conjugated A02110HRP WB
Anti-RFP Tag Mouse Mab (9D1) A02120 WB
Anti-RFP Tag Mouse Mab (9D1), HRP Conjugated A02120HRP WB
Anti-S Tag Mouse Mab (9T10) A02130 WB
Anti-S Tag Mouse Mab (9T10), HRP Conjugated A02130HRP WB
Anti-T7 Tag Mouse Mab (6D4) A02150 WB
Anti-T7 Tag Mouse Mab (6D4), HRP Conjugated A02150HRP WB
Anti-Trx Tag Mouse Mab (14D4) A02160 WB
Anti-Trx Tag Mouse Mab (14D4), HRP Conjugated A02160HRP WB
Anti-CBP Tag Mab (12H5) A02190 WB
Anti-CBP Tag Mab (12H5), HRP Conjugated A02190HRP WB
Anti-TAP Tag Mab (4H2) A02200 WB
Anti-TAP Tag Mab (4H2), HRP Conjugated A02200HRP WB
Anti-Avi-Tag Mab (5G11) A02210 WB
Anti-Avi-Tag Mab (5G11), HRP Conjugated A02210HRP WB
Anti-SRT-Tag Mab (11G3) A02220 WB
Anti-SRT-Tag Mab (11G3), HRP Conjugated A02220HRP WB
Anti-mStrawberry Mab (4C9) A02240 WB
Anti-mStrawberry Mab (4C9), HRP Conjugated A02240HRP WB
Anti-EYFP-Tag Mab (10T3) A02250 WB
Anti-EYFP-Tag Mab (10T3), HRP Conjugated A02250HRP WB
Anti-mOrange Mab (9A10) A02260 WB
Anti-mOrange Mab (9A10), HRP Conjugated A02260HRP WB
Anti-AmCyan Mab (8T2) A02270 WB
Anti-AmCyan Mab (8T2), HRP Conjugated A02270HRP WB
Anti-ECFP-Tag Mab (6B11) A02280 WB
Anti-ECFP-Tag Mab (6B11), HRP Conjugated A02280HRP WB
Anti-EBFP Mab (8B5) A02290 WB
Anti-EBFP Mab (8B5), HRP Conjugated A02290HRP WB
Anti-Nano-Tag9 Mab (11T3) A02300 WB
Anti-Nano-Tag9 Mab (11T3), HRP Conjugated A02300HRP WB


How to choose an epitope?

* The best known tag is poly-histidine, which is based upon the affinity of nickel for four or more consecutive histidine residues. It is popular because of its ability to purify under denaturing conditions, ease of reuse, and reasonable price.

* Tags that use antibody-based purification formats (such as DDDDK, HA, HSV, c-Myc) offer higher levels of purity, but the antibody resins are expensive to produce and cannot be reused easily. This is of particular use in high-throughput screening, or where proteins are expressed at low levels.

* Other tags with cost-effective purification resins are MBP and GST. These tags are approximately 40 times the size of metal affinity tags, so they may interfere with the structure or function of the fusion partner. However, tags such as MBP and GST can improve solubility, which is especially important when expressing proteins at high levels in prokaryotes as they have more primitive posttranslational folding and processing machinery than eukaryotes.

* Epitope tags can also be used for immobilization or attachment, to investigate protein interactions. If a purification resin is loaded with protein, then it can be used as ‘bait’ to capture binding partners (immunoprecipitation). In this case, the specifcity of the resin becomes crucial to avoid interference from contaminants.

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