New York – Aug 30th, 2018 – Creative Biogene, a reliable biotechnology products and service provider, offering Green Fluorescent Protein (GFP) – a type of the Reporter Genes – which is a powerful molecular imaging tool that is commonly applied in biology, genetics, chemistry, and medicine. Furthermore, with years of experience and in-depth research, scientists in Creative Biogene already established a large number of GFP Reporter Cell Lines that can be suitable for a various application.
As a protein in the jellyfish Aequorea Victoria that exhibits green fluorescence when exposed to light, the structure of the Green fluorescent protein is identical to that of any protein, therefore, it has a multilayer structure and a variety of chemical interaction methods. The basic or primary structure of GFP is a 238 amino acid chain, weighing about 27,000 atomic mass units, and only about 4 amino acids directly produce any fluorescent effect. The secondary structure is a series of spirals and pleats caused by hydrogen bonds in the chain, while the tertiary structure is a barrel made of 11 plates, covered with a spiral. There is a chromophore located in the center, which is an amino acid responsible for the short-chain changes in luminescence. The barrel structure keeps the chromophore away from the solvent, allowing GFP to fluoresce under any condition and denaturation of proteins by heat and pH.
Biologists utilize GFP as a marker protein to study embryonic and fetal cells during development, as it can attach and label another fluorescent protein, enabling scientists to observe the presence of specific proteins in organic structures. With DNA recombination technology, scientists combine the GFP gene with another gene that produces the protein they want to study and then insert the complex into the cell. It is postulated that the cell expresses the target gene if the cell produces green fluorescence. In addition, GFP is utilized to label specific organelles, cells, and tissues. Due to the GFP gene is heritable, the progeny of the marker entity also exhibit green fluorescence.
GFP can also be used to label successful transgenes as it is not toxic to transgenic animals, and can be monitored rapidly and non-invasively by near-ultraviolet or blue-light illumination. ‘GFP can be fused to a gene or tissue-specific promoter, expression can be monitored when a given tissue is subjected to certain promoter responsive stimuli,’ said Wendy Wilson, Marketing Director of Creative Biogene. ‘GFP has been proved to be valuable tools for detecting tumors or diseased tissues in the whole animal or human patients.’
About Creative Biogene
Founded in 2005, Creative Biogene provides scientists with innovative products and services that can be applied in gene discovery, regulation to greatly improve the experiment performance, further promoting genomics and proteomics-based biological research. Creative Biogene is a well-recognized industry leader with more than 10 years experience, holding a leadership position in the global market.
Company Name: Creative Biogene
Contact Person: Wendy Wilson
Email: Send Email
Address:45-1 Ramsey Road
State: New York
Country: United States