Profacgen, a world leader in serving science, recently updated its stable cell line construction services, especially for those when a large amount of recombinant protein is required. This update is aimed for proving reproducible and cost-effective protein production for scientists worldwide.
Profacgen uses lentiviral vector for efficient gene delivery and to achieve high integration rate. Limited dilution and antibiotics screening are then applied to select for high-yield, genetically homogenous stable expression monoclones. When fluorescent protein is fused to the target protein, the company also uses flow cytometry to screen for positive clones. Profacgen also offers the generation of Tet-ON inducible stable cell lines for inducible expression of its customers’ target gene. Doxycycline can then be used to induce the expression of their target gene.
With the features of high yield and reproducibility, high cell line stability, one-stop service, and service fully customizable, the newly updated stable cell line construction service in Profacgen provides new chances for scientists in need of quality service both for research use and industrial production.
To ensure integration of its customers’ tagert gene and stable or inducible expression of the protein, Profacgen also provides PCR, qRT-PCR, microscopy, flow cytometry analysis and stability test based on the customers’ specific needs.
To get more detailed information about the recently updated stable cell line construction service in Profacgen, please visit https://www.profacgen.com/stable-cell-line-construction.htm.
Profacgen provides process development and cGMP manufacturing services for biological active ingredients. We have ready access to state-of-the-art biopharmaceutical product development and manufacturing facilities and our staff scientists have demonstrable expertise in all aspects of biopharmaceutical manufacturing, from facility design and operation to the ability to provide best advice relating to product development. We also save our clients a great deal of time with bioprocess scale-up services. Our fermentation laboratories are equipped with state-of-the-art fermentors and bioreactors that enable us to express protein in large scale. Our customers can choose to enter into the protein purification pipeline at any stage depending on their needs.
Company Name: Profacgen
Contact Person: Ellen Burns
Email: Send Email
Address:45-1 Ramsey Road
State: New York
Country: United States