Abbkine recently releases Mitochondrion/Nuclei Extraction Kits, you can visit Abbkine category of Sampling kit for detailed products information.
ExKine™ Mitochondrion Isolation Kit for Cultured Cells contains Lysis Buffer A (5×), Lysis Buffer B, Lysis Buffer C, Storage Buffer.
Mitochondria is a double-membrane-bound organelle found in most eukaryotic cells. The function of mitochondria is to provide cellular energy. Moreover, mitochondria are involved in other tasks, such as signaling, cellular differentiation, and cell death, as well as maintaining control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders, cardiacdysfunction, heart failure and autism. Mitochondria may play an important role in these cellular processes.
Abbkine ExKine™ Mitochondrion Extraction Kit (Cultured Cells) enables rapid and crude isolation of of intact mitochondria from cultured mammalian cells. The kit offers two protocols for the separation of mitochondria from cytosolic components relying on differential centrifugation. First protocol utilizes a reagent-based method allowing multiple samples to be processed concurrently. The second protocol uses traditional Dounce homogenization and provides approximately two-fold more mitochondria.
Mitochondrial (M) and Cytosolic (C) fractions were analyzed by WB using cytochrome C antibody (cat#ABM40191), VDAC1 antibody (cat#ABP53121) and COX IV antibody (cat#ABP53121).
The Cultured Cells Mitochondrial Extraction Kit has below features:
1. Isolate intact mitochondria from 20 million cells in less than 1 hour.
2. Process several samples simultaneously using the reagent-based method, or obtain highest possible yield with the Dounce homogenization method.
3. Isolated mitochondria is compatible with almost any downstream application.
Please click here for more Extraction Kits.
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