New York, Oct 22nd, 2018 – Creative Bioarray, a reliable and leading biotechnology company focusing on offering researchers with normal human and animal cells, cell culture medium and reagents etc., released a professional primary cell culture guide. This guide includes solutions for scientists to solve several issues in the process of cell culture. Besides, the high-quality cells and reagents offered through the comprehensive culture system also are available to accelerate lab research.
Cell types most frequently found in primary cell culture are epithelial cells, endothelial Cells, immortalized Cells, and fibroblasts etc. Primary cell cultures more closely mimic the physiological state of cells in the body and produce more relevant data that represents the vital system. Primary cultures consist of freshly derived cells derived from living organisms and maintain growth in vitro. Meanwhile, primary cells can be classified according to their isolated genus, as well as species or tissue type.
Once adapted to in vitro culture conditions, primary cells undergo a limited predetermined number of cell divisions before entering senescence. Differing from tumor cell lines cultured in medium containing 10% to 20% serum, primary cell cultures are critical and require optimized growth conditions, including the addition of tissue-specific cytokines and growth factors for propagation in serum-free or low serum growth media.
At present, primary cell cultures are mainly applied as in vitro tools for preclinical and research biology studies, such as intercellular and intercellular communication studies, developmental biology, and elucidation of disease mechanisms such as cancer, Parkinson’s disease, and diabetes. In history, researchers have used immortalized cell lines in studies related to tissue function. However, the application of cell lines containing total mutations and chromosomal abnormalities provides a poor indicator of normal cell phenotype and early disease progression. The use of primary cells that are only maintained for a short period of time in vitro now serves as the best representative of the major functional components of the tissue (in vivo) from which they are derived.
There are some challenges while using the primary cells, one of which the primary cells culturers have to meet is the limited cell accessibility due to donor tissue supply problems, cell isolation/purification difficulties, quality assurance and consistency, and contamination risks. Comparability is also a serious problem with primary cell use and stems from the differences between the reagents used and the procedures performed by individual scientists and laboratories to isolate and culture primary cells.
‘Primary cells are cells taken directly from living tissue (eg, biopsy material) and established for in vitro growth. These cells undergo very few population doublings and therefore represent more of the major functional components of the tissue from which they are derived than continuous tumor or artificial immortalized human cell lines, therefore, representing a more representative model of the state of the body.’ said Chelsea Clark, marketing director of Creative Bioarray, she also claimed that ‘we designed this primary cell culture guide to show new researchers the basic principles of primary cells and some practical cultural skills.’
About Creative Bioarray
Founded in 2005, Creative Bioarray is dedicated to offering scientists with innovative biotechnology products and services to greatly improve the experiment performance and accelerate life science research. Creative Bioarray is an industry leader owning world reputation with more than 10 years experience and support researchers worldwide.
Company Name: Creative Bioarray
Contact Person: Chelsea Clark
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Address:45-1 Ramsey Road Shirley
City: Long Island City
State: New York
Country: United States