One of the most easily measured features of apoptotic cells is the break-up of the genomic DNA by cellular nucleases. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) is a method for detecting DNA fragmentation by labeling the 3′-hydroxyl termini in the double-strand DNA breaks generated during apoptosis. The TUNEL assay relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that catalyzes the addition of dUTPs that are labeled with fluorescein.
Abbkine Cell Apoptosis (DNA Fragmentation), Green Fluorescence TUNEL Apoptosis Detection Kit contains TdT Enzyme, Equilibration Buffer (5X), Label Nucleotide Mix.
The Abbkine TUNEL Apoptosis Detection Kit (Green Fluorescence) relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that catalyzes the addition of dUTPs that are labeled with fluorescein.
The featured Cell Apoptosis (DNA Fragmentation) Kit has below features:
1. Optimized assay protocol, suitable for fluorescence microplate reader, fluorescence microscope, or flow cytometer.
2. Signal can be easily detected at the popular FITC channel (Ex/Em = 490nm/520 nm).
More cell status detection products at celluar assay kit.
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