New York, April 25, 2019 With manufacturing and quality control of high standards, Creative Biolabs, now offers comprehensive CAR-T Gene Packaging & Delivery services, including virus based transfection, electroporation transfection, transposon based transfection.
Chimeric antigen receptor (CAR)-engineered T cell therapy with higher specificity and lower toxicity shows remarkable ability to eliminate various kinds of tumors, raising more and more attention in cancer treatment field. Aiming at genetically modifying T cells with designed CAR, Creative Biolabs flexibly adopts versatile methodologies to transfect CAR construction into T cells, which include virus based transfection, electroporation transfection, transposon based transfection.
The major advantages of viral vectors are relative ease of manufacture and production and capacity to stably integrate genetic material into the host genome. In Creative Biolabs, gamma retroviral vectors and lentiviral vectors are two common vectors used in Virus Based Transfection. Gamma-retrovirus (simple retrovirus, retroviral, MLV) contains a reverse transcriptase to enable the integration of artificial genes into the host genome in a stable status while lentivirus vectors is capable of mediating potent transduction and stable expression into dividing and non-dividing cells both in vitro and in vivo.
Electroporation can be used with most cell types, especially T cells, yielding a high frequency of both stable transformation and transient gene expression, which is characterized by easy operation and its applicability for transient and stable transfection of T cells. Moreover, electric transfection is easy and rapid to transfect a large number of T cells in a short time. In Creative Biolabs, electroporation is optimized by distributing the electrical pulse equally among the cells and maintaining a stable pH and modifying the pulse and field strength parameters to reduce the loss.
Transposons are a non-virial alternative for stable gene transfer, including two main vectors, namely Sleeping Beauty (SB) and PiggyBac (PB). The SB system is a safer alternative to viral vectors due to its non-pathogenic origin, inherently low enhancer activity, and minimal epigenetic modifications at the insertion site, while PB system shows a large cargo capacity and more efficient transposition activity in preclinical studies. Scientists in Creative Biolabs optimize the transposon transfection to have higher capacity of carrying target DNA. Further, efficiency of gene movement and higher safety than the virial vectors make CARs production more reliable.
As a world-renowned service provider for immunotherapy, Creative Biolabs has one of the world’s largest collection of CAR products of different generations targeting various biomarkers. In addition to transfection services, Creative Biolabs also offers high-quality custom service covering the entire CAR-T therapy development process to best suit the technical, program and budget requirements to greatly assist your research, preclinical investigation and clinical stage development. More information can be reached at www.creative-biolabs.com/car-t.
As a global company, Creative Biolabs has more than 200 talented and well-trained scientists located in different continents working closely with partners from the entire world to develop and produce medicines of tomorrow. Specifically, the established leading experts in TCR and CAR T&NK cell immune therapy development offer the one-stop custom services that cover the entire new drug development pipeline, and an exclusive line of ready-to-use TCR and CAR T&NK cell construction products, such as virus packaging, purification, expansion and titer determination kits. Furthermore, a unique unparalleled CAR construction and production platform has been built up for all four CAR generations.
Company Name: Creative Biolabs
Contact Person: Candy Swift
Email: Send Email
Address:45-1 Ramsey Road
State: New York
Country: United States