It is quite important to determine the structural, chemical and functional properties of proteins for biopharmaceutical and life science researches. It has been verified by numerous research and development (R&D) process for initial characterization of a protein’s biochemical activity and functionality, alongside physical instability testing. Among them, a vital parameter to monitor throughout this process is protein molecular weight.
Proteins have distinct and intricate structures due to the differences in the amino acids charge, functional group composition, size, shape, etc. If the composition and sequence of amino acids in a linear chain are known, protein molecular weight can be accurately predicted based on the known molecular weights of the amino acids.
Proteins structure is roughly classified into Primary Structures, Secondary Structures and Tertiary Structures.
Protein molecular weight is a key parameter to confirm the line since the primary structure is the most basic foundation. Therefore, getting to know the unmodified protein molecular weight can assist in initial assessments of the biomolecule’s functionality, for example, the gene regulation, metabolic regulation, enzyme catalysis, immunological responses and the bond and transfer of small molecules.
By years of efforts and practice, Creative Proteomics has developed and enhanced a comprehensive list of technical platforms to conduct protein identification better, including:
• Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)
• Gel permeation chromatography (GPC)
• Size exclusion chromatography (SEC)
• Matrix Assisted Laser Desorption Ionization Mass Spectrometry (MALDI-MS)
• Electrospray Ionization Mass Spectrometry (ESI-MS)
• Liquid Chromatography with Mass Spectrometry Detection (LC-MS)
Determining Protein Molecular Weight with Creative Proteomics
Creative Proteomics is one of the world’s leading service companies in proteomics, metabolomics, glycomics, and bioinformatics analysis fields. The company offers a wide selection of molecular weight analysis techniques, such as Mass Spectrometry (MS), and accuracy of measurement over a wide molecular weight range, with minimal amount of sample. With its well-established platforms, it promises accurate identification and monitoring of low intensity isoforms. Furthermore, any difference between theoretical and measured mass may be identified as potential modifications on the proteins.