New York, USA – April 23, 2021 – With years of experience in offering professional and high-quality products for academic use and pharmaceutical industries, Creative Proteomics announces HPLC-tMS analysis service for metabolomics profiling. Metabolomics is an omics method to analyze all metabolites of a certain biological component or cell quantitatively and qualitatively under a specific physiological or pathological condition. It can be used for early disease diagnosis, drug target discovery, and disease mechanism research. Metabolomics mainly includes untargeted metabolomics and targeted metabolomics.
1. Untargeted metabolomics: In a set of experimental and control samples, it detects metabolites with statistically significant changes in abundance, identifies their structure, and reveals the correlation between the discovered metabolites and physiological or pathological conditions.
2. Targeted metabolomics: The research target is a certain type of compound or a compound related to a certain metabolic pathway. The target ion pair can be selected for high-sensitivity quantitative analysis using MRM mode.
S-adenosylmethionine (SAM) is a biochemical methyl donor that is catalyzed by methionine adenosyltransferase with ATP involved. SAM demethylates under the action of methyltransferase to form S-adenosylhomocysteine (SAH). Finally, SAH is hydrolyzed to remove adenosine and converted to homocysteine.
SAM and SAH are widely present in various organisms. As important physiologically active substances, they participate in the methylation of body metabolism and are closely related to many key bodily functions including gene regulation, hormone activity, nerve conduction development and detoxification. They show a wide range of therapeutic effects, have good efficacy in the treatment of liver dysfunction, depression, arthritis, and other diseases, and are important indicators of Alzheimer’s disease (AD), Parkinson’s disease (PD), systemic lupus erythematosus, cancer and many other diseases.
Creative Proteomics has extensive experience in providing advanced metabolomics services. In a collaboration with a research group in the Department of Molecular Pharmacology, Albert Einstein College of Medicine, we provided services to quantitatively analyze SAM and SAH levels in plasma and liver samples. The SAM methyl cofactor and its SAH methyltransferase product were analyzed in plasma. In brief, plasma samples were spiked with an internal standard solution in 0.1% formic acid and then treated with 70% perchloric acid. Following acid extraction, supernatants were analyzed by HPLC-tMS in the multiple reaction monitoring (MRM) setting. For liver SAM and SAH, HPLC was used to analyze acid extracted rat liver samples. Both SAH and SAM were characterized by a maximum absorption at 256 nm and eluted at 11.39 and 12.76 min, respectively; such detection was linear across a 5-140 pmol range.
“Our specialized service is designed to provide a versatile and reliable platform for metabolomics analysis. We have advanced equipment, professional metabolomics, and a bioinformatics team to ensure the quality of service. Over the past ten years, Creative Proteomics has helped clients complete hundreds of projects, from project design and conducting experiments to the interpretation of results and bioinformatics analysis,” stated Creative Proteomics’s executive VP.
About Creative Proteomics
Creative Proteomics has gradually grown into an integrated service provider with metabolomics, lipidomics, and bioinformatics analysis services to researchers in pharmaceutical, biotechnology, agriculture, and nutrition industries, as well as academic and government organizations. With a continued focus on quality, Creative Proteomics is proud to satisfy our clients’ needs both at home and abroad, covering more than 50 countries and districts.