New York, USA – May 18, 2021 – CD Genomics is one of the leading service providers in the area of genomic sequencing and data analysis. The company can now offer fast and reliable circRNA sequencing services to support research concerning gene regulation, mechanisms of disease, and many other fields that involve circRNA.
Comprehensive detection of circRNAs from high-throughput transcriptome data is an initial and crucial step to study their biogenesis and function. The high-throughput sequencing of rRNA/linear RNA-depleted RNA in combination with computational tools has led to the identification of thousands of new circRNAs and analysis of their linear host transcripts quantitatively in diverse organisms.
Unlike miRNAs and other small RNAs, circRNAs are not easily separated from other types of RNAs by size or electrophoretic mobility. Consequently, they are generally retained in rRNA-depleted libraries and enriched in libraries treated with RNase R that only digest linear RNA.
“Viral noncoding RNAs have acquired increasing prominence as important regulators of infection and mediators of pathogenesis. Circular RNAs (circRNAs) generated by backsplicing events have been identified in several oncogenic human DNA viruses,” noted by researchers at the University of Pittsburgh, “we used RNase R-treated samples for library preparation and subsequent circRNA sequencing by CD Genomics. And we found four putative circRNA backsplice junctions (BSJs) were identified from the Merkel cell polyomavirus early region.” He continued, “which was extremely useful for clarifying the regulatory functions of circRNAs.”
“CD Genomics’s circRNA sequencing can be used to profile circRNAs across a wide dynamic range, which covers the identification of known and novel circRNAs. And we can also help researchers explore novel biomarkers and circRNAs regulatory networks,” said Dr. Charlie of CD Genomics.
“The first and foremost step of the circRNA sequencing workflow is constructing circRNA sequencing library, wherein our highly experienced expert team ensures that every step of rRNA depletion, linear RNA digestion, and strand-specific library preparation goes smoothly. We highly value quality management, following every procedure to ensure confident and unbiased results.” Dr. Charlie added, “we spare no effort to deliver the best service, high-quality data with flexibility, and bioinformatics analysis based on customers’ requirements.”
CD Genomics is providing the circRNA sequencing service that utilizes the latest Illumina platforms for a fast, economical, and accurate characterization. The bioinformatics analysis includes but not limited to:
• Raw data quality control
• Reference-based mapping
• CircRNA identification and annotation
• Known and novel circRNA expression analysis
• Differential expression analysis, enrichment analysis, and functional analysis
• Origin analysis of circRNA including target miRNA prediction and circRNA-miRNA interaction network analysis
About CD Genomics
CD Genomics is one of the top genomic research service providers, providing reliable services to pharmaceutical and biotech companies as well as academia and government agencies. At CD Genomics, experienced specialists will work closely with researchers to determine the optimal sequencing strategy based on the specific research focus. With state-of-the-art sequencing platforms and years of industry experience, CD Genomics guarantees high-quality data and integrated bioinformatics analyses.