Cellular senescence is a phenomenon in which normal cells cease to divide. The senescent cells can no longer replicate, but they still maintain metabolically active and stain positive for senescence-associated beta-galactosidase activity, which is considered to be a biomarker of cellular senescence. Cellular senescence can be achieved by the activation of oncogenes and cell-cell fusion, DNA damage in response to elevated reactive oxygen species (ROS), rather than relying solely on the number of cellular divisions. During normal aging, the number of senescent cells in tissues increases significantly.
Abbkine Cell Senescence Kit (β-Galactosidase Assay) contains below components:
• X-gal (lyophilized)
• Fixation Buffer (10×)
• Reagent A
• Reagent B
• Reagent C
The Abbkine Cell Senescence Detection/Staining Kit (β-Galactosidase Assay) provides reagents for detecting the senescent cells or tissues based on the up-regulation of senescence-associated β-galactosidase (SA-β-Gal) activity at pH 6 during aging. The aging of cells or tissues can be observed under a common optical microscope. SA-β-Gal is present only in senescent cells and is undetectable in pre-senescent, quiescent, tumor or immortal cells.
Our Cellular Senescence Assay Kit (SA-β-gal Staining) has below features:
• Optimized assay protocol, suitable for cellular senescence detection of senescent cells or tissues.
• Only stain senescent cells and does not stain presencent cells, quiescent cells, immortal cells or tumor cells.
Note: The β-galactosidase staining solution is toxic and corrosive to the human body. Please be careful when handling it, and pay attention to effective protection. Avoid contacting with the human body or inhalation directly.
More cell status detection Kit at celluar assay kit.
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