New York, US–Jan 28, 2019 With advanced technologies and experienced researchers, Creative Biolabs, a professional service provider for chimeric antigen receptor (CAR)-engineered T cell immune cell therapy, introduces its comprehensive list of CAR cell in vitro assay services.
In recent years, CAR modified T cell or natural killer (NK) cell has been becoming an attractive immunotherapy for treating many types of cancer. A full range of validation assay services are required to verify the solid success of CAR-T construction and superior function. At Creative Biolabs, scientists will professionally perform multiple evaluations on the post-transfection CAR-T cells to align with customer’s specific T cells engineered objective.
CAR Expression tests
CAR Expression tests at different stages of the whole CAR-T cell therapy are indispensable for obtaining high-quality CAR-T cells in CAR-T In Vitro Testing. Creative Biolabs can offer CAR expression and stability test service based on flow cytometric analysis so as to evaluate our CAR expression level on T cells or NK cells with high precision. Meanwhile, western blotting analysis system for total CAR expression is also provided.
Cytokine Release Test
The level of cytokines released by modified T cells is one of the indicators reflecting the functional activity of T cells in vivo to verify the integrity of immune functions of the CAR-T cells. Creative Biolabs provides CellRapeutics™ cytokine release test such as Enzyme-Linked ImmunoSorbent Assay (ELISA) for detecting the secretion of Interferon gamma (IFNγ), Interleukin-2 (IL-2) and other cytokines released by CAR-T cells or CAR-NK cells against tumor.
In Vitro Cytotoxicity Test
In the CAR-T cell therapy, since CAR-T cells antigen-independent proliferation may happen due to acute on-target/on-tumor toxicities (cytokine release syndrome, tumor lysis syndrome) or on-target/off-tumor toxicities , in vitro cytotoxicity test of the CAR-T cells and the possible adverse effects is the most important validation step for the safety consideration. In Creative Biolabs, the possible toxicity of CAR engrafted T cell or NK cell to normal cells can be evaluated via cytotoxicity analysis, or CARs can be coexpressed with an appropriate suicide gene to regulate this potential cytotoxicity and kill the cancer cells without influencing the normal ones.
CAR Cell Proliferation Test
Scientists in Creative Biolabs have developed the second and the third generation CAR products to enhance the persistence of CAR-T cells or -NK cells proliferation. Meanwhile, this activation and proliferation process can also be evaluated via our different proliferation assay approaches such as MTT, MTS, Cell Counting Kit-8 (CCK-8), 5, 6-carboxyfluorescein diacetate succinimidyl ester (CFSE) in combination with flow cytometric analysis, [3H] thymidine incorporation and other techniques. At the same time, cytokines concentration (e.g. IL-2) assay is also available for determining T cell proliferation.
“Besides these four tests mentioned above, viability and bio-distribution analysis and efficacy test are also available, conducted by utilizing appropriate animal models, designing comprehensive research practices and handling high-quality in vivo studies.” said by a scientist engaged in Creative Biolabs.
As a world-renowned service provider for immunotherapy, Creative Biolabs has one of the world’s largest collection of products and services for CAR-T Therapy Development. Please visit https://www.creative-biolabs.com/car-t for more information.
About Creative Biolabs
As a global company, Creative Biolabs has more than 200 talented and well-trained scientists located in different continents working closely with partners from the entire world to develop and produce medicines of tomorrow. Specifically, the established leading experts in TCR and CAR T&NK cell immune therapy development offer the one-stop custom services that cover the entire new drug development pipeline, and an exclusive line of ready-to-use TCR and CAR T&NK cell construction products, such as virus packaging, purification, expansion and titer determination kits. Furthermore, a unique unparalleled CAR construction and production platform has been built up for all four CAR generations.
Company Name: Creative Biolabs
Contact Person: Candy Swift
Email: Send Email
Address:45-1 Ramsey Road
State: New York
Country: United States