SHIRLEY, NY – May 24, 2019 – Creative Proteomics, a leading supplier in metabolomics services, has recently upgraded its protein identification and peptides analysis services to researchers. Theprotein identification service ranges include peptide mass fingerprinting (PMF), de novo peptides/proteins sequencing service, N-terminal sequencing, shotgun protein identification, which are based on the most powerful platforms and techniques in this field with unrivaled accuracy and sensitivity.
As mass spectrometry advances, the identification of proteins and peptides is no longer cumbersome and time-consuming. To shorten the duration of protein identification and improve the accuracy of protein sequencing, Creative Proteomics has long been working to optimize its mass spectrometry technology platform. They developed a comprehensive protein identification pipeline from sample preparation, protein separation and purification, followed by mass analysis, and finally to systematic bioinformatics analysis.
Creative Proteomics’ shotgun proteomics uses bottom-up proteomics techniques to study the whole proteins in complex mixtures, such as serum, urine and cell lysates etc. It utilizes high performance liquid chromatography (HPLC) technology combined with mass spectrometry (MS) technology. The most striking feature of shotgun protein identification is its ability to simultaneously identify and quantify a wide range of proteins at the same time with minimal protein separation needed.
Creative Proteomics provides N-terminal sequencing analysis of therapeutic proteins, monoclonal antibodies and protein vaccines by Edman and mass spectrometry. In protein EdmanN-terminal sequencing, the protein was first modified with phenyl isothiocyanate (PITC). Subsequently, the derivatized terminal amino acid is removed by acid cleavage in the form of a phenylhydantoin (PTH) derivative, and the new α-amino group on the next amino acid is now available for the next round of reaction with PITC. Therefore, the protein sequence is being analyzed by the severe reaction of PITC addition, and one cleavage of one PTH at a time for analysis. Suppose the reaction is completely effective, the method can sequence the entire amino acid from the N-terminal end. Although less efficient than mass spectrometers, N-terminal Edman sequencing still has the advantage of protein analysis, while other analytical methods are not readily available.
De Novo Peptides/Proteins Sequencing Service is important to study the biological function of the peptide/protein. However, a complete characterization of peptides/proteins, including post-translational modifications (PTMs), sequence mutations and variants, is very challenging. There are two approaches to determine the sequence of peptide/protein by mass spectrometry: database search and de novo sequencing. Database search approach compares acquired mass spectra to a database of known protein sequences to identify the protein sequences. De novo sequencing is a process in which amino acid sequences are directly interpreted from tandem mass spectra without the assistance of a database.
Peptide Mass Fingerprinting (PMF) is an analytical technique for identifying the unknown protein. Proteins to be identified is first digested into peptides, whose absolute mass can be accurately analyzed by a mass spectrometer, such as MALDI-TOF or ESI-TOF. Creative Proteomics provides Peptide Mass Fingerprinting (PMF) analysis and ions searching against a database for rapid identification of proteins.
“Creative Proteomics is a professional biotech company specializing in providing protein identification services. Based on years’ experience and knowledge in the application of proteomics analysis, Creative Proteomics can provide fast turnaround, clear concise written reports, and custom service to help customers resolve your analytical and technical challenges.” said Dr. Charlie.
About Creative Proteomics
Creative Proteomics, as an experienced proteomics, metabolomics, glycomics, and bioinformatics services supplier, has been equipped with advanced technologies to meet the growing needs of researchers and scientists in proteomics and metabolomics related researches.
Company Name: Creative Proteomics
Contact Person: Dora West
Email: Send Email
Address:45-1 Ramsey Road
State: New York
Country: United States