The Protein Expression Market is projected to reach USD 2,850.5 Million by 2022 from USD 1,654.0 Million in 2017, at a CAGR of 11.5% to reach.
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Emergence of microfluidics
Microfluidics is among the most important and advanced techniques used in protein expression studies and research. Microfluidic antibody capture (MAC) chip is a novel technology which is used to measure the expression level of different proteins and their phosphorylation at the single-cell level.
Advancements in the microfluidics technology have provided robust solutions for the analysis and evaluation of tumorous and non-tumorous cells. Microfluidics chips are easy to use and provide reliable and efficient isolation of cells even from a small sample volume. Citing the potential of microfluidics in protein expression analysis, various research studies are being performed for evaluating the performance of microfluidics chips in the quantification of protein expression level. Moreover, innovative and advanced applications of microfluidics in protein expression research will help overcome challenges related to protein analysis; this technology is thus expected to offer significant growth opportunities to the protein expression market during the forecast period.
Contamination—a major challenge in protein purification
Contamination is one the major challenges during the large-scale production of pharmaceutical proteins and vaccines. It can be caused due to a number of contaminants, such as aerosol fall-out during culture incubation contact with media or solutions and unsealed dishes & culture plates. During the large-scale production of proteins, biological contaminants may cause changes in the physiological conditions of media, which leads to whole batch contamination.
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In mammalian protein expression, CHO cell lines are used for the production of tissue plasminogen activator, pulmozyme, herceptin, and erythropoietin. However, there is a greater risk of contamination while using CHO cell lines. CHO cell lines may be contaminated from mycoplasma and thus hamper the manufacturing process of proteins. Maintaining contamination-free conditions is, therefore, one of the key challenges in research laboratories for the Protein Expression market.
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